Reverse transcriptase
| Reverse transcriptase (RNA-dependent DNA polymerase) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 | |||||||||
| Identifiers | |||||||||
| Symbol | RVT_1 | ||||||||
| Pfam | PF00078 | ||||||||
| Pfam clan | CL0027 | ||||||||
| InterPro | IPR000477 | ||||||||
| PROSITE | PS50878 | ||||||||
| SCOP | 1hmv | ||||||||
| SUPERFAMILY | 1hmv | ||||||||
| CDD | cd00304 | ||||||||
| |||||||||
| RNA-directed DNA polymerase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC number | 2.7.7.49 | ||||||||
| CAS number | 9068-38-6 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Reverse transcriptase (RT) යනු RNA template එකකින් reverse transcription ක්රමවේදය හරහා complementary DNA (cDNA) නිපදවීමේදී භාවිතාවන එන්සයිමයක් වේ.
ඉතිහාසය
[සංස්කරණය]University of Wisconsin–Madison හි Howard Temin විසින් reverse transcriptases සොයාගත් අතර [2] 1970 දී MIT හි David Baltimore සිදුකරන ලද ස්වාදීන පර්යේෂණයක දී RNA tumour වෛරස දෙකක් වන murine leukemia virus සහ Rous sarcoma virus වලින් reverse transcriptase වෙන් කර ගන්නා ලදී.[3] එම සොයාගැනීමට 1975 දී ඔවුන් දෙදෙනා හට Nobel Prize in Physiology or Medicine (Renato Dulbecco සමඟ) හීමිවිය.
ආශ්රිත
[සංස්කරණය]
- cDNA library
- DNA polymerase
- msDNA
- Reverse transcribing virus
- RNA polymerase
- Telomerase
- Retrotransposon marker
මූලාශ්ර
[සංස්කරණය]- ^ PDB: 3KLF; Tu X, Das K, Han Q, Bauman JD, Clark AD, Hou X, Frenkel YV, Gaffney BL, Jones RA, Boyer PL, Hughes SH, Sarafianos SG, Arnold E (October 2010). "Structural basis of HIV-1 resistance to AZT by excision". Nature Structural & Molecular Biology. 17 (10): 1202–9. doi:10.1038/nsmb.1908. PMC 2987654. PMID 20852643.
- ^ Temin HM, Mizutani S (June 1970). "RNA-dependent DNA polymerase in virions of Rous sarcoma virus". Nature. 226 (5252): 1211–3. doi:10.1038/2261211a0. PMID 4316301.
- ^ Baltimore D (June 1970). "RNA-dependent DNA polymerase in virions of RNA tumour viruses". Nature. 226 (5252): 1209–11. doi:10.1038/2261209a0. PMID 4316300.
භාහිර සබැඳි
[සංස්කරණය]- RNA+Transcriptase at the US National Library of Medicine Medical Subject Headings (MeSH)
- animation of reverse transcriptase action and three reverse transcriptase inhibitors
- Molecule of the month සංරක්ෂණය කළ පිටපත 2020-04-09 at the Wayback Machine (September 2002) at the RCSB PDB
- HIV Replication 3D Medical Animation. (Nov 2008). Video by Boehringer Ingelheim.
- Goodsell DS. "Molecule of the Month: Reverse Transcriptase (Sep 2002)". Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (PDB). සම්ප්රවේශය 2013-01-13.
- PDBe-KB provides an overview of all the structure information available in the PDB for Human immunodeficiency virus Reverse transcriptase